Laminar Air flow is an important Instrument for Biotechnology, Pathology, and many Biology based laboratory. For a normal biology laboratory or in a pathology laboratory in a rural area, it is sometimes difficult to buy the instrument due to its cost. The design of the present Portable Table-top Laminar Air Flow Machine represents the idea of a low-cost handy Laminar Air Flow. It contains two compartments, small air circulating chamber and large, working area. The air circulation is maintained in the smaller sub chamber containing air-pressure creating DC high-speed Fan, dust protecting micro-filter, and HEPA filter. The large chamber contains working stage, transparent outer door, UV light, and LED light. The air coming through the dust-protecting micro-filter and then through DC fans create air pressure that releases through HEPA filter uniformly into the working compartment by horizontal air pressure from back to front. This air pressure can be monitored by the manometer placed outside the chamber. The dimension of the present designed machine is 24 x 28 x 28 inch with a working compartment dimension of 24 x 19 x 23 inch. The weight is approximately 12 Kg. The electric consumption is 154 watts during 100 percent working condition. The efficacy has been confirmed through flame tests and media contamination tests.